Evaluate dyslipidemia prevalence and its association with insulin resistance in a cohort of apparently healthy subjects. A cross-sectional study was conducted with 1,179 donors ages 35 to 65 years. The sample population was comprised of 71% men, with an average age of 44 +/- 7. Clinical records, anthropometric data, lipid profile, fasting glycaemia, and insulin levels were obtained. Prevalence of hypertriglyceridemia was 57.3%, C-HDL under normal limits was 52.4%, and hypercholesterolemia was 48.7%. In addition, 36.8% of the obese individuals (as measured by waist perimeter) had hypertriglyceridemia/hypoalphalipoproteinemia, 35.2% had mixed dyslipidemia, and 33.4% had hypertriglyceridemia. Patterns of dyslipidemia were higher in subjects diagnosed with insulin resistance. Insulin resistance associated with hypertriglyceridemia and hypoalphalipoproteinemia was common among our studied population. However, a significant proportion of cases of apparent healthy individuals continue to go undiagnosed.